Biosynthesis of Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) from Different 4-Hydroxybutyrate Precursors by New Wild-Type Strain Cupriavidus necator IBP/SFU-1

The study addresses the growth of new wild-type strain Cupriavidus necator IBP/SFU-1 and synthesis poly(3-hydroxybutyrate-co-4-hydroxybutyrate) (P(3HB-co-4HB) on media containing fructose three different precursors 4HB (ε-caprolactone 1,4-butanediol 1,6-hexanediol). It was found that ε-caprolactone is best precursor for P(3HB-co-4HB) copolymers. By varying concentration number doses added into bacterial culture, it possible to find conditions ensured copolymers with contents (from 3–5 22.4 mol.%). physicochemical properties were investigated depending proportions monomers. effect monomers manifested in a certain decrease weight-average molecular weight (Mw) (272–353 kDa), number-average (Mn) (47–67 kDa) samples, an increase polydispersity (5.09–6.71) compared P(3HB). crystallinity degree decreased increasing fraction units 72 59%, as content increased from 0 In addition, affected temperature parameters (melting point, glass transition temperature, crystallization thermal degradation temperature).